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Bayesian phylogenetic analyses of WGS knowledge from EPICC cohort had been carried out applying BEAST21. The model we employed was just like the CRC phylogenetic analyses reported before2. We produced an enter XML file for BEAST2 with BEAUti. The model and parameters we utilised are as under: as all clones have been sampled simultaneously, we set all tip dates as 0; as to the substitution model, we employed the GTR design and set the Gamma Class Rely to 4; we utilised the “Relaxed Clock Exponential” as clock model; As for prior with the calm clock amount signify, we employed 4.
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